The GDC is a shared facility to provide its users a managed laboratory for DNA/RNA/protein extraction and quantification, DNA amplification, sequencing, and genotyping as well as data analysis mostly for non-model organism. A special focus is on high throughput technologies to cope with the increasing number of individuals and markers needed. Since 2012 we also offer library preparation for different Next Generation Sequencing platforms. Below a list of the main instruments available at the GDC.
Library preparation for Next (New) Generation Sequencing
We provide library preparation support for Illumina and PacBio sequencing platforms. If you want to prepare libraries for other NGS platforms (e.g. 454 or Iontorrent), please contact us.
For RADtaq sequencing we have primers and adapters in stock. We have 24 different barcoded P1 Adapter and several barcoded P2 Adapter available which we offer in small aliquots.
The following sizing and size selection tools are available:
DNA / RNA / protein extraction
Fragment analysis (e.g. Microsatellites, AFLP)
Gene expression analysis
Copy number variation
CpG methylation analysis
Three data analysis computers with various applications are available for our users. The software packages include GeneMapper, Assay Design, Sequencher, CLC Genomics Workbench, Geneious, 454 Newbler, and SMRT portal.
We also maintain a Sequencher (DNA analysis and assembly software) and Geneious network license that is available for PC and Mac users.
Users are also able to access the GDC internal computing environment. Currently the GDC has the following two Linux computers for computational biology and data storage:
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